Bioluminescent assay of femtomole levels of estrone and estradiol.

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Nicolas JC, Boussioux AM, Boularan AM, Descomps B, Crastes de Paulet A

Bioluminescent assay of femtomole levels of estrone and estradiol.

Anal Biochem. 1983 Nov;135(1):141-5.

PubMed ID
6584048 [ View in PubMed
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Abstract

A rapid and very sensitive enzymatic assay of estrogens was developed using the transhydrogenase reaction of the human estradiol-17 beta dehydrogenase and bioluminescence for the quantification of NADH accumulated during the first reaction. The assay requires two steps: first, the addition of transhydrogenase buffer, and a few minutes later the addition of the bioluminescence reagent. The method can determine 0.1 to 50 pg of estrogens in the assay, in 1 h, with a coefficient of variation of 5%.

DrugBank Data that Cites this Article

Drug Targets
DrugTargetKindOrganismPharmacological ActionActions
NADHAldo-keto reductase family 1 member C3ProteinHumans
Unknown
Not AvailableDetails