Sevoflurane modulates the activity of glyceraldehyde 3-phosphate dehydrogenase.

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Swearengin TA, Fibuch EE, Seidler NW

Sevoflurane modulates the activity of glyceraldehyde 3-phosphate dehydrogenase.

J Enzyme Inhib Med Chem. 2006 Oct;21(5):575-9.

PubMed ID

17194030 [ View in PubMed

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Abstract

The mechanism of inhalation anesthesia remains to be fully elucidated. While certain neuronal membrane proteins are considered sites of action, cytosolic proteins may also be targets. We hypothesize that inhaled anesthetics may act via glyceraldehyde 3-phosphate dehydrogenase (GAPDH), which has recently been shown to participate in neuronal inhibition. We examined the effects of sevoflurane, a halogenated ether anesthetic, on the catalytic and fluorescence properties of GAPDH. Initial rates of oxidoreductase activity decreased approximately 30% at saturating levels of sevoflurane. NADH-stimulated oxidoreductase activity (25 microM NADH; 0.8mM NAD+) increased with sevoflurane. Sevoflurane quenched tryptophan fluorescence emission and increased polarization. Additionally, sevoflurane increased the susceptibility of GAPDH to thermal denaturation suggesting an effect on conformation. Our findings warrant further research on sevoflurane's effect on GAPDH and indicate that this approach may lead to delineation of a novel contribution to the mechanism of anesthesia.

DrugBank Data that Cites this Article

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
NADH	Glyceraldehyde-3-phosphate dehydrogenase	Protein	Humans	Unknown	Not Available	Details