Nucleotide sequence of the gene for human factor IX (antihemophilic factor B).

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Yoshitake S, Schach BG, Foster DC, Davie EW, Kurachi K

Nucleotide sequence of the gene for human factor IX (antihemophilic factor B).

Biochemistry. 1985 Jul 2;24(14):3736-50.

PubMed ID

2994716 [ View in PubMed

]

Abstract

Two different human genomic DNA libraries were screened for the gene for blood coagulation factor IX by employing a cDNA for the human protein as a hybridization probe. Five overlapping lambda phages were identified that contained the gene for factor IX. The complete DNA sequence of about 38 kilobases for the gene and the adjacent 5' and 3' flanking regions was established by the dideoxy chain termination and chemical degradation methods. The gene contained about 33.5 kilobases of DNA, including seven introns and eight exons within the coding and 3' noncoding regions of the gene. The eight exons code for a prepro leader sequence and 415 amino acids that make up the mature protein circulating in plasma. The intervening sequences range in size from 188 to 9473 nucleotides and contain four Alu repetitive sequences, including one in intron A and three in intron F. A fifth Alu repetitive sequence was found immediately flanking the 3' end of the gene. A 50 base pair insert in intron A was found in a clone from one of the genomic libraries but was absent in clones from the other library. Intron A as well as the 3' noncoding region of the gene also contained alternating purine-pyrimidine sequences that provide potential left-handed helical DNA or Z-DNA structures for the gene. KpnI repetitive sequences were identified in intron D and the region flanking the 5' end of the gene. The 5' flanking region also contained a 1.9-kb HindIII subfamily repeat. The seven introns in the gene for factor IX were located in essentially the same position as the seven introns in the gene for human protein C, while the first three were found in positions identical with those in the gene for human prothrombin.

DrugBank Data that Cites this Article

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Antihemophilic factor, human recombinant	Coagulation factor IX	Protein	Humans	Yes	Cofactor	Details
Lonoctocog alfa	Coagulation factor IX	Protein	Humans	Yes	Cofactor	Details
Moroctocog alfa	Coagulation factor IX	Protein	Humans	Yes	Cofactor	Details

Polypeptides

Name	UniProt ID
Coagulation factor IX	P00740	Details