Colorimetric endpoint assay for enzyme-catalyzed iodide ion release for high-throughput screening in microtiter plates.

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Kurtovic S, Jansson R, Mannervik B

Colorimetric endpoint assay for enzyme-catalyzed iodide ion release for high-throughput screening in microtiter plates.

Arch Biochem Biophys. 2007 Aug 15;464(2):284-7. Epub 2007 Apr 24.

PubMed ID

17490601 [ View in PubMed

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Abstract

Efforts are being made to engineer enzymes with enhanced activities against haloalkanes, a toxicologically important class of compounds widely used and frequently occurring in the environment. Here we describe a facile, inexpensive, and robust method for the screening of libraries of mutated enzymes with iodoalkane substrates. Iodide formed in the enzymatic reaction is oxidized to iodine, which in the presence of starch gives blue color that can be measured at 610nm or scored with the human eye. The assay can be performed with enzymes in crude cell lysates in 96-wells microtiter plates. Expression clones of several glutathione transferases showed diverse activities with different iodoalkanes, and a mutant library of human glutathione transferase A1-1 expressed variants with enhanced substrate selectivities.

DrugBank Data that Cites this Article

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Glutathione	Glutathione S-transferase Mu 2	Protein	Humans	Unknown	Not Available	Details