Demonstration of bile acid transport across the mammalian peroxisomal membrane.

Article Details

Citation

Copy to clipboard

Visser WF, van Roermund CW, Ijlst L, Waterham HR, Wanders RJ

Demonstration of bile acid transport across the mammalian peroxisomal membrane.

Biochem Biophys Res Commun. 2007 Jun 1;357(2):335-40. Epub 2007 Mar 26.

PubMed ID

17416343 [ View in PubMed

]

Abstract

It is well established that peroxisomes play a crucial role in de novo bile acid biosynthesis. The primary bile acids resulting from peroxisomal beta-oxidation are conjugated to either glycine or taurine in the peroxisomal lumen by a bile acid aminotransferase (BAT). These conjugated bile acids are subsequently secreted into the bile. In this paper we show that the export of glycine- and taurine-conjugated bile acids from mammalian peroxisomes proceeds via specific transporter. The transport activity of this protein was detected by reconstitution of peroxisomal membrane proteins in liposomes and measuring the uptake of radiolabeled substrates into these proteoliposomes. The transporter was further characterized using this assay, which led to the identification of DIDS as an inhibitor of the peroxisomal bile-acid transporter, and allowed us to establish some kinetic parameters for the transport activity.

DrugBank Data that Cites this Article

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Glycine	Bile acid-CoA:amino acid N-acyltransferase	Protein	Humans	Unknown	Substrate	Details