Conformational changes and catalysis by alcohol dehydrogenase.
Article Details
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Plapp BV
Conformational changes and catalysis by alcohol dehydrogenase.
Arch Biochem Biophys. 2010 Jan 1;493(1):3-12. doi: 10.1016/j.abb.2009.07.001. Epub 2009 Jul 5.
- PubMed ID
- 19583966 [ View in PubMed]
- Abstract
As shown by X-ray crystallography, horse liver alcohol dehydrogenase undergoes a global conformational change upon binding of NAD(+) or NADH, involving a rotation of the catalytic domain relative to the coenzyme binding domain and the closing up of the active site to produce a catalytically efficient enzyme. The conformational change requires a complete coenzyme and is affected by various chemical or mutational substitutions that can increase the catalytic turnover by altering the kinetics of the isomerization and rate of dissociation of coenzymes. The binding of NAD(+) is kinetically limited by a unimolecular isomerization (corresponding to the conformational change) that is controlled by deprotonation of the catalytic zinc-water to produce a negatively-charged zinc-hydroxide, which can attract the positively-charged nicotinamide ring. The deprotonation is facilitated by His-51 acting through a hydrogen-bonded network to relay the proton to solvent. Binding of NADH also involves a conformational change, but the rate is very fast. After the enzyme binds NAD(+) and closes up, the substrate displaces the hydroxide bound to the catalytic zinc; this exchange may involve a double displacement reaction where the carboxylate group of a glutamate residue first displaces the hydroxide (inverting the tetrahedral coordination of the zinc), and then the exogenous ligand displaces the glutamate. The resulting enzyme-NAD(+)-alcoholate complex is poised for hydrogen transfer, and small conformational fluctuations may bring the reactants together so that the hydride ion is transferred by quantum mechanical tunneling. In the process, the nicotinamide ring may become puckered, as seen in structures of complexes of the enzyme with NADH. The conformational changes of alcohol dehydrogenase demonstrate the importance of protein dynamics in catalysis.
DrugBank Data that Cites this Article
- Drug Enzymes
Drug Enzyme Kind Organism Pharmacological Action Actions NADH Alcohol dehydrogenase [NADP(+)] Protein Humans UnknownSubstrateDetails NADH Alcohol dehydrogenase 1A Protein Humans UnknownSubstrateDetails NADH Alcohol dehydrogenase 1B Protein Humans UnknownSubstrateDetails NADH Alcohol dehydrogenase 4 Protein Humans UnknownSubstrateDetails NADH Alcohol dehydrogenase 6 Protein Humans UnknownSubstrateActivatorDetails NADH Alcohol dehydrogenase class 4 mu/sigma chain Protein Humans UnknownSubstrateDetails NADH Alcohol dehydrogenase class-3 Protein Humans UnknownSubstrateDetails