Characterization, cDNA cloning, and functional expression of mouse ileal sodium-dependent bile acid transporter.

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Saeki T, Matoba K, Furukawa H, Kirifuji K, Kanamoto R, Iwami K

Characterization, cDNA cloning, and functional expression of mouse ileal sodium-dependent bile acid transporter.

J Biochem. 1999 Apr;125(4):846-51.

PubMed ID

10101301 [ View in PubMed

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Abstract

Mouse ileal sodium dependent bile acid transporter (ISBT) was characterized using isolated enterocytes. Only enterocytes from the most distal portion showed Na+-dependent [3H]taurocholate uptake. Northern blot analysis using a probe against mouse ISBT revealed the expression of mouse ISBT mRNA to be restricted to the distal ileum. The Km and Vmax for Na+-dependent [3H]taurocholate transport into isolated ileocytes were calculated as 27 microM and 360 pmol/mg protein/min, respectively. Uptake of [3H]taurocholate was inhibited by N-ethylmaleimide. We have cloned ISBT cDNA from mouse ileum. The cDNA included the entire open reading frame coding 348 amino acid protein with seven hydrophobic segments and two N-glycosylation sites. COS-7 cells transfected with the expression vector containing this cDNA expressed Na+-dependent [3H]taurocholate uptake activity with a Km of 34 microM.

DrugBank Data that Cites this Article

Drug Transporters

Drug	Transporter	Kind	Organism	Pharmacological Action	Actions
Cholic Acid	Ileal sodium/bile acid cotransporter	Protein	Humans	Unknown	Substrate Inhibitor Inducer	Details
Deoxycholic acid	Ileal sodium/bile acid cotransporter	Protein	Humans	Unknown	Inhibitor	Details
Taurocholic acid	Ileal sodium/bile acid cotransporter	Protein	Humans	Unknown	Substrate Inducer	Details
Ursodeoxycholic acid	Ileal sodium/bile acid cotransporter	Protein	Humans	Unknown	Inhibitor	Details