Characterization of cloned mouse Na+/taurocholate cotransporting polypeptide by transient expression in COS-7 cells.

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Saeki T, Takahashi N, Kanamoto R, Iwami K

Characterization of cloned mouse Na+/taurocholate cotransporting polypeptide by transient expression in COS-7 cells.

Biosci Biotechnol Biochem. 2002 May;66(5):1116-8.

PubMed ID

12092825 [ View in PubMed

]

Abstract

The mouse Na+/taurocholate cotransporting polypeptide transiently expressed in COS-7 cells caused sodium-dependent uptake of [3H]taurocholic acid with Km and Vmax values of 18 microM and 102 pmol/mg protein/min, respectively. This Km value is comparable to that for rat NTCP and higher than that for human NTCP. Substrate specificity was evaluated by measuring inhibitory effects of unlabeled bile acids on [3H]taurocholic acid transport.

DrugBank Data that Cites this Article

Drug Transporters

Drug	Transporter	Kind	Organism	Pharmacological Action	Actions
Cholic Acid	Ileal sodium/bile acid cotransporter	Protein	Humans	Unknown	Substrate Inhibitor Inducer	Details
Deoxycholic acid	Ileal sodium/bile acid cotransporter	Protein	Humans	Unknown	Inhibitor	Details
Taurocholic acid	Ileal sodium/bile acid cotransporter	Protein	Humans	Unknown	Substrate Inducer	Details
Ursodeoxycholic acid	Ileal sodium/bile acid cotransporter	Protein	Humans	Unknown	Inhibitor	Details