Molecular characterization of human and rat organic anion transporter OATP-D.
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Adachi H, Suzuki T, Abe M, Asano N, Mizutamari H, Tanemoto M, Nishio T, Onogawa T, Toyohara T, Kasai S, Satoh F, Suzuki M, Tokui T, Unno M, Shimosegawa T, Matsuno S, Ito S, Abe T
Molecular characterization of human and rat organic anion transporter OATP-D.
Am J Physiol Renal Physiol. 2003 Dec;285(6):F1188-97.
- PubMed ID
- 14631946 [ View in PubMed]
- Abstract
We have isolated and characterized a novel human and rat organic anion transporter subtype, OATP-D. The isolated cDNA from human brain encodes a polypeptide of 710 amino acids (Mr 76,534) with 12 predicted transmembrane domains. The rat clone encodes 710 amino acids (Mr 76,821) with 97.6% amino acid sequence homology with human OATP-D. Human and rat OATP-D have moderate amino acid sequence homology with LST-l/rlst-1, the rat oatp family, the prostaglandin transporter, and moatl/MOAT1/KIAA0880/OATP-B. Phylogenetic tree analysis revealed that OATP-D is branched in a different position from all known organic anion transporters. OATP-D transports prostaglandin E1 (Km 48.5 nM), prostaglandin E2 (Km 55.5 nM), and prostaglandin F2,, suggesting that, functionally, OATP-D encodes a protein that has similar characteristics to those of the prostaglandin transporter. Rat OATP-D also transports prostaglandins. The expression pattern of OATP-D mRNA was abundant mainly in the heart, testis, brain, and some cancer cells. Immunohistochemical analysis further revealed that rat OATP-D is widely expressed in the vascular, renal, and reproductive system at the protein level. These results suggest that OATP-D plays an important role in translocating prostaglandins in specialized tissues and cells.
DrugBank Data that Cites this Article
- Drug Transporters
Drug Transporter Kind Organism Pharmacological Action Actions Alprostadil Solute carrier organic anion transporter family member 3A1 Protein Humans UnknownSubstrateInhibitorDetails Aminohippuric acid Solute carrier organic anion transporter family member 3A1 Protein Humans UnknownInhibitorDetails Dinoprost tromethamine Solute carrier organic anion transporter family member 3A1 Protein Humans UnknownNot Available Details Dinoprostone Solute carrier organic anion transporter family member 3A1 Protein Humans UnknownSubstrateDetails Iloprost Solute carrier organic anion transporter family member 3A1 Protein Humans UnknownSubstrateDetails Methotrexate Solute carrier organic anion transporter family member 3A1 Protein Humans UnknownSubstrateDetails Taurocholic acid Solute carrier organic anion transporter family member 3A1 Protein Humans UnknownInhibitorDetails