Aspirin enhances multidrug resistance gene 1 expression in human Molt-4 T lymphoma cells.

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Flescher E, Rotem R, Kwon P, Azare J, Jaspers I, Cohen D

Aspirin enhances multidrug resistance gene 1 expression in human Molt-4 T lymphoma cells.

Anticancer Res. 2000 Nov-Dec;20(6B):4441-4.

PubMed ID

11205285 [ View in PubMed

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Abstract

BACKGROUND: We recently found that aspirin induces the expression of P-glycoprotein (P-gp), a protein mediating drug resistance, in human prostate cancer cells. The purpose of this study was to evaluate the effect of aspirin on the expression of P-gp in a different human cancer type, i.e., T lymphoma. Furthermore, we analyzed this effect at the level of the gene encoding P-gp, MDR1, and of the transcription factor (NF-IL6), regulating this gene. MATERIALS AND METHODS: NF-IL6 was assayed by the electrophoretic mobility shift assay, MDR1 mRNA was assayed by the reverse transcriptase polymerase chain reaction (RT-PCR), and P-gp was assayed by Western blotting. RESULTS: aspirin, at plasma attainable levels, induced NF-IL6 DNA-binding activity, and increased MDR1 mRNA expression (by up to 140%), as well as the expression of P-gp, in Molt-4 cells. CONCLUSIONS: This study suggests that treatment with aspirin induces a cellular signal culminating in the enhancement of P-gp expression in T lymphoma Molt-4 cells.

DrugBank Data that Cites this Article

Drug Transporters

Drug	Transporter	Kind	Organism	Pharmacological Action	Actions
Acetylsalicylic acid	P-glycoprotein 1	Protein	Humans	Unknown	Substrate Inducer Modulator	Details