The zinc containing pro-apoptotic protein siva interacts with the peroxisomal membrane protein pmp22.

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Nestler M, Martin U, Hortschansky P, Saluz HP, Henke A, Munder T

The zinc containing pro-apoptotic protein siva interacts with the peroxisomal membrane protein pmp22.

Mol Cell Biochem. 2006 Jul;287(1-2):147-55. Epub 2006 May 9.

PubMed ID

16683188 [ View in PubMed

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Abstract

Host answers to pathogen attacks define the course of pathogenic events and decide about the fate of the host organism. Infection with coxsackievirus B3 (CVB3) can induce severe myocarditis and pancreatitis. The interplay between host factors and virus components is crucial for the fate of the infected host. As we have shown before, expression of the pro-apoptotic host protein Siva is significantly increased after CVB3 infection, and infected cells are removed by programmed cell death. Analysis of Siva expressed in Escherichia coli revealed that this protein binds three zinc ions, suggesting a rather complex three-dimensional structure. By screening a human heart cDNA library we found a new interaction partner of Siva. The peroxisomal membrane protein PMP22 may be involved in the host response against CVB3. Previous investigations showed that Siva interacts with the cytoplasmic C-terminus of CD27, a member of the tumor necrosis factor receptor group, and transmits an apoptotic signal. With the help of directed two-hybrid assays we determined the N-terminal part of Siva as the binding region for CD27.

DrugBank Data that Cites this Article

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Zinc	Apoptosis regulatory protein Siva	Protein	Humans	Unknown	Not Available	Details
Zinc acetate	Apoptosis regulatory protein Siva	Protein	Humans	Unknown	Not Available	Details
Zinc chloride	Apoptosis regulatory protein Siva	Protein	Humans	Unknown	Binder	Details
Zinc sulfate, unspecified form	Apoptosis regulatory protein Siva	Protein	Humans	Unknown	Binder	Details

Polypeptides

Name	UniProt ID
Apoptosis regulatory protein Siva	O15304	Details