In vitro interaction between ceruloplasmin and human serum transferrin.

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Ha-Duong NT, Eid C, Hemadi M, El Hage Chahine JM

In vitro interaction between ceruloplasmin and human serum transferrin.

Biochemistry. 2010 Dec 7;49(48):10261-3. doi: 10.1021/bi1014503. Epub 2010 Nov 9.

PubMed ID
21049900 [ View in PubMed
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Abstract

The thermodynamics of the interactions of serum apotransferrin (T) and holotransferrin (TFe(2)) with ceruloplasmin (Cp), as well as those of human lactoferrin (Lf), were assessed by fluorescence emission spectroscopy. Cp interacts with two Lf molecules. The first interaction depends on pH and mu, whereas the second does not. Dissociation constants were as follows: K(11Lf) = 1.5 +/- 0.2 muM, and K(12Lf) = 11 +/- 2 muM. Two slightly different interactions of T or TFe(2) with Cp are detected for the first time. They are both independent of pH and mu and occur with 1:1 stoichiometry: K(1T) = 19 +/- 7 muM, and K(1TFe2) = 12 +/- 4 muM. These results can improve our understanding of the probable process of the transfer of iron from Cp to T in iron and copper transport and homeostasis.

DrugBank Data that Cites this Article

Drug Targets
DrugTargetKindOrganismPharmacological ActionActions
Ferrous ascorbateCeruloplasminProteinHumans
Unknown
Not AvailableDetails
Ferrous ascorbateSerotransferrinProteinHumans
Unknown
Not AvailableDetails
Ferrous fumarateCeruloplasminProteinHumans
Unknown
Not AvailableDetails
Ferrous fumarateSerotransferrinProteinHumans
Unknown
Not AvailableDetails
Ferrous gluconateCeruloplasminProteinHumans
Unknown
Not AvailableDetails
Ferrous gluconateSerotransferrinProteinHumans
Unknown
Not AvailableDetails
Ferrous glycine sulfateCeruloplasminProteinHumans
Unknown
Not AvailableDetails
Ferrous glycine sulfateSerotransferrinProteinHumans
Unknown
Not AvailableDetails
Ferrous succinateCeruloplasminProteinHumans
Unknown
Not AvailableDetails
Ferrous succinateSerotransferrinProteinHumans
Unknown
Not AvailableDetails
IronCeruloplasminProteinHumans
Unknown
Not AvailableDetails
IronSerotransferrinProteinHumans
Unknown
Not AvailableDetails