Structure of lactate dehydrogenase inhibitor generated from coenzyme.

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Biellmann JF, Lapinte C, Haid E, Weimann G

Structure of lactate dehydrogenase inhibitor generated from coenzyme.

Biochemistry. 1979 Apr 3;18(7):1212-7.

PubMed ID
218616 [ View in PubMed
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Abstract

Two inhibitors of lactate dehydrogenase generated during NADH storage have been isolated by chromatography. One is a dimer of the dinucleotide where the AMP moiety is unmodified. The other is also generated from NAD+ in the presence of a high concentration of phosphate ions at alkaline pH. This inhibitor was proved to be the addition compound of one phosphate group to position C-4 of the nicotinamide ring of NAD+ by NMR spectroscopy, enzymatic cleavage, and dissociation to NAD+ at neutral pH. This compound is a competitive inhibitor with respect to NAD+ in the presence of the lactate dehydrogenase with a Ki of 2 X 10(-7) M. The interaction of this inhibitor with lactate dehydrogenase is discussed relative to the structure of this enzyme.

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