Validation of enantioseparation and quantitation of an active metabolite of abrocitinib in human plasma.
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Tripathy S, Wentzel D, Wan XK, Kavetska O
Validation of enantioseparation and quantitation of an active metabolite of abrocitinib in human plasma.
Bioanalysis. 2021 Oct;13(19):1477-1486. doi: 10.4155/bio-2021-0128. Epub 2021 Oct 4.
- PubMed ID
- 34601943 [ View in PubMed]
- Abstract
Aims: A chiral HPLC-MS/MS method for quantitation of an active metabolite (M2) of abrocitinib was validated in human plasma. Methods: Protein precipitation extraction and normal phase LC with baseline separation of five analytes (abrocitinib; isomeric metabolites M1, M2, M3 and M4) were achieved followed by mass spectrometric quantitation of M2 using positive-mode APCI. Results: With a 5-5000 ng/ml assay range using 100 mul K2EDTA aliquot, the assay provided short (17-min) runtime and robust separation up to approximately 330 injections on one column. Interday and intraday accuracy ranged from -6.80% to 13.4%; between-day and within-day precision was
