Spatiotemporal expression of the creatine metabolism related genes agat, gamt and ct1 during zebrafish embryogenesis.

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Wang L, Zhang Y, Shao M, Zhang H

Spatiotemporal expression of the creatine metabolism related genes agat, gamt and ct1 during zebrafish embryogenesis.

Int J Dev Biol. 2007;51(3):247-53.

PubMed ID

17486546 [ View in PubMed

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Abstract

Glycine amidinotransferase (AGAT or GATM), guanidinoacetate methyltransferase (GAMT) and creatine transporter (CT1) are three proteins involved in the synthesis and uptake of creatine. The expression patterns of these three genes were examined in zebrafish embryos by whole mount in situ hybridization followed by histological sectioning. Expression of agat first appeared in the yolk syncytial layer (YSL) at the gastrula stage and was progressively up regulated during gastrulation. As development proceeds, agat was expressed in the mature somites during the segmentation stage and in the liver at 48 hpf. gamt showed a similar expression pattern to that of agat during embryogenesis. It was first detected in the center of the yolk from the cleavage to the gastrula stage. At the bud stage, its expression shifted to the YSL. gamt was also transiently expressed in the mature somites from 16 hpf to 24 hpf and became strongly expressed in the liver and in epithelial cells of the gut at 48 hpf. ct1 was initially uniformly expressed from the cleavage to the early segmentation stage; it was then strongly expressed in all the somites till 30 hpf and in the gut of 48 hpf embryos. However, ct1 transcripts also appeared in the central nervous system during the segmentation stage, but not in the YSL, the yolk or the liver. Our data reveal for the first time distinct and unique patterns of expression of the creatine metabolism genes agat, gamt and ct1 during zebrafish embryogenesis.

DrugBank Data that Cites this Article

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Creatine	Guanidinoacetate N-methyltransferase	Protein	Humans	Yes	Product of	Details
Creatine	Sodium- and chloride-dependent creatine transporter 1	Protein	Humans	Yes	Not Available	Details
Glycine	Glycine amidinotransferase, mitochondrial	Protein	Humans	Unknown	Substrate	Details
Phosphocreatine	Guanidinoacetate N-methyltransferase	Protein	Humans	Yes	Product of	Details
Phosphocreatine	Sodium- and chloride-dependent creatine transporter 1	Protein	Humans	Yes	Not Available	Details