Membrane expression of annexin I is enhanced by calcium and TPA in cultured human keratinocytes.

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Sato-Matsumura KC, Matsumura T, Nakamura H, Sawa H, Nagashima K, Koizumi H

Membrane expression of annexin I is enhanced by calcium and TPA in cultured human keratinocytes.

Arch Dermatol Res. 2000 Oct;292(10):496-9.

PubMed ID
11142771 [ View in PubMed
]
Abstract

Annexin I plays an important role in the process of keratinization as a component of the cornified envelope. To elucidate the function of annexin I in keratinization, we investigated the effects of calcium, epinephrine, hydrocortisone, and 12-O-tetradecanoyl phorbol 13-acetate (TPA) on the expression and localization of annexin I in cultured human keratinocytes. Normal human keratinocytes were cultured in serum-free culture medium (0.15 mM calcium) until 70% confluence. After incubation with a higher concentration of calcium (1.8 mM), TPA (100 nM), epinephrine (50 microM), or hydrocortisone (10 microM) for 24 h, the expression of annexin I protein and mRNA was examined using immunofluorescence, Western blot, and Northern blot techniques. Immunofluorescence microscopy showed increased membrane staining of annexin I by calcium, which was inhibited by the addition of epinephrine or hydrocortisone. Western blotting confirmed elevated annexin I on the cell membrane. It was increased in the cell membrane fraction, but not in the cytosol fraction. Interestingly, the mRNA level of annexin I was slightly reduced after incubation with calcium, whereas TPA upregulated both membrane expression and the mRNA level. Secretion of annexin I was increased by TPA but inhibited by calcium. Because calcium and TPA are known to promote keratinization, our data suggest that annexin I expression on the cell membrane is involved in the process of keratinization.

DrugBank Data that Cites this Article

Drug Targets
DrugTargetKindOrganismPharmacological ActionActions
HydrocortisoneAnnexin A1ProteinHumans
Yes
Agonist
Details
Hydrocortisone aceponateAnnexin A1ProteinHumans
Yes
Not AvailableDetails
Hydrocortisone acetateAnnexin A1ProteinHumans
Yes
Not AvailableDetails
Hydrocortisone butyrateAnnexin A1ProteinHumans
Yes
Not AvailableDetails
Hydrocortisone cypionateAnnexin A1ProteinHumans
Yes
Not AvailableDetails
Hydrocortisone phosphateAnnexin A1ProteinHumans
Yes
Not AvailableDetails
Hydrocortisone probutateAnnexin A1ProteinHumans
Yes
Not AvailableDetails
Hydrocortisone valerateAnnexin A1ProteinHumans
Yes
Not AvailableDetails