Expression of annexin I in freshly isolated human epidermal cells and in cultured keratinocytes.
Article Details
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Serres M, Viac J, Comera C, Schmitt D
Expression of annexin I in freshly isolated human epidermal cells and in cultured keratinocytes.
Arch Dermatol Res. 1994;286(5):268-72.
- PubMed ID
- 8060156 [ View in PubMed]
- Abstract
Annexin I belongs to a newly characterized family of intracellular proteins involved in the regulation of the production of inflammatory lipid mediators such as prostaglandins and leucotrienes. Annexin I (named p35, lipocortin I or calpactin II) was initially described as a protein inducible by glucocorticoids. In the skin, the role of annexins has still not been elucidated. In the study reported here we investigated the expression of annexin I both in freshly isolated epidermal cells and in cultured keratinocytes using immunofluorescence, FACS analysis and immunoblotting techniques. Using epidermal cells freshly isolated from normal skin, annexin I was detected by double immunostaining mainly in basal and suprabasal keratinocytes. Langerhans cells isolated from Ficoll gradient were faintly stained compared with keratinocytes. Annexin I was also highly expressed in keratinocytes maintained in culture in a serum-free medium without hydrocortisone. By confocal microscopy, annexin I was shown to be mainly localized in the cytoplasm of the cells. The protein was characterized by Western blot and immunoprecipitation as a 35-kDa protein in freshly isolated epidermal cells and cultured keratinocytes. Results from in vivo studies confirmed the presence of annexin I in the basal and suprabasal layers of normal human skin with modified reactivity patterns in hyperproliferative lesions.
DrugBank Data that Cites this Article
- Drug Targets
Drug Target Kind Organism Pharmacological Action Actions Betamethasone phosphate Annexin A1 Protein Humans UnknownAgonistDetails Budesonide Annexin A1 Protein Humans UnknownSubstrateDetails Clobetasol propionate Annexin A1 Protein Humans UnknownInducerDetails Cortisone acetate Annexin A1 Protein Humans UnknownInducerDetails Hydrocortisone Annexin A1 Protein Humans YesAgonistDetails Hydrocortisone aceponate Annexin A1 Protein Humans YesNot Available Details Hydrocortisone acetate Annexin A1 Protein Humans YesNot Available Details Hydrocortisone butyrate Annexin A1 Protein Humans YesNot Available Details Hydrocortisone cypionate Annexin A1 Protein Humans YesNot Available Details Hydrocortisone phosphate Annexin A1 Protein Humans YesNot Available Details Hydrocortisone probutate Annexin A1 Protein Humans YesNot Available Details Hydrocortisone valerate Annexin A1 Protein Humans YesNot Available Details Methylprednisolone Annexin A1 Protein Humans UnknownAgonistDetails Prednisolone phosphate Annexin A1 Protein Humans UnknownLigandDetails