The cysteine-desulfurase IscS promotes the production of the rhodanese RhdA in the persulfurated form.

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Forlani F, Cereda A, Freuer A, Nimtz M, Leimkuhler S, Pagani S

The cysteine-desulfurase IscS promotes the production of the rhodanese RhdA in the persulfurated form.

FEBS Lett. 2005 Dec 19;579(30):6786-90. Epub 2005 Nov 21.

PubMed ID
16310786 [ View in PubMed
]
Abstract

After heterologous expression in Escherichia coli, the Azotobacter vinelandii rhodanese RhdA is purified in a persulfurated form (RhdA-SSH). We identified l-cysteine as the most effective sulfur source in producing RhdA-SSH. An E. coli soluble extract was required for in vitro persulfuration of RhdA, and the addition of pyridoxal-5'-phosphate increased RhdA-SSH production, indicating a likely involvement of a cysteine desulfurase. We were able to show the formation of a covalent complex between IscS and RhdA. By combining a time-course fluorescence assay and mass spectrometry analysis, we demonstrated the transfer of sulfur from E. coli IscS to RhdA.

DrugBank Data that Cites this Article

Drug Targets
DrugTargetKindOrganismPharmacological ActionActions
Pyridoxal phosphateCysteine desulfurase, mitochondrialProteinHumans
Unknown
Cofactor
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