Biotransformation of benzothiazole derivatives by the Pseudomonas putida strain HKT554.

Article Details

Citation
Copy to clipboard

El-Bassi L, Iwasaki H, Oku H, Shinzato N, Matsui T

Biotransformation of benzothiazole derivatives by the Pseudomonas putida strain HKT554.

Chemosphere. 2010 Sep;81(1):109-13. doi: 10.1016/j.chemosphere.2010.07.024. Epub 2010 Aug 6.

PubMed ID
20692014 [ View in PubMed
]
Abstract

We examined the biotransformation of benzothiazole derivatives (BTHs) by an axenic microbial culture. A Gram-negative bacterium, tentatively named as strain HKT554 and identified as Pseudomonas putida, was able to transform not only benzothiazole and 2-mercaptobenzothiazole but also 2-methylthiobenzothiazole, which was previously reported as the dead-end product of wastewater treatment. GC/MS analysis of the solid-phase extract of the culture broth showed the formation of 2-(3H)-benzothiazolone/2-hydroxybenzothiazole from benzothiazole. By transposon mutagenesis, a mutant library containing ca. 5000 insertion mutants was constructed from the P. putida strain HKT554. Analysis of the disrupted gene from one of the mutants showing BTHs transformation deficiency revealed that the knocked-out gene was naphthalene dioxygenase. To our knowledge, this is the first report on the biotransformation of BTHs by Gram-negative bacteria.

DrugBank Data that Cites this Article

Drugs