Redistribution of carbon flux in Torulopsis glabrata by altering vitamin and calcium level.

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Liu L, Li Y, Zhu Y, Du G, Chen J

Redistribution of carbon flux in Torulopsis glabrata by altering vitamin and calcium level.

Metab Eng. 2007 Jan;9(1):21-9. Epub 2006 Aug 12.

PubMed ID

17008113 [ View in PubMed

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Abstract

Manipulation of cofactor (thiamine, biotin and Ca(2+)) levels as a potential tool to redistribute carbon flux was studied in Torulopsis glabrata. With sub-optimization of vitamin in fermentation medium, the carbon flux was blocked at the key node of pyruvate, and 69 g/L pyruvate was accumulated. Increasing the concentrations of thiamine and biotin could selectively open the valve of carbon flux from pyruvate to pyruvate dehydrogenase complex, the pyruvate carboxylase (PC) pathway and the channel into the TCA cycle, leading to the over-production of alpha-ketoglutarate. In addition, the activity of PC was enhanced with Ca(2+) present in fermentation medium. By combining high concentration's vitamins and CaCO(3) as the pH buffer, a batch culture was conducted in a 7-L fermentor, with the pyruvate concentration decreased to 21.8 g/L while alpha-ketoglutarate concentration increased to 43.7 g/L. Our study indicated that the metabolic flux could be redistributed to overproduce desired metabolites with manipulating the cofactor levels. Furthermore, the manipulation of vitamin level provided an alternative tool to realize metabolic engineering goals.

DrugBank Data that Cites this Article

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Biotin	Pyruvate carboxylase, mitochondrial	Protein	Humans	Unknown	Cofactor	Details
Pyruvic acid	Pyruvate carboxylase, mitochondrial	Protein	Humans	Unknown	Not Available	Details