[Restraint stress down-regulates L-Arg/NOS/NO pathway of platelet and aortic intima in rats].

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Cui YY, Tang CS, Geng B

[Restraint stress down-regulates L-Arg/NOS/NO pathway of platelet and aortic intima in rats].

Beijing Da Xue Xue Bao. 2006 Jun 18;38(3):231-5.

PubMed ID

16778961 [ View in PubMed

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Abstract

OBJECTIVE: To investigate alteration and cross link of the aortic intima and platelet endogenous L-Arg/NOS/NO pathway induced by water immersion restraint (WIR) stress. METHODS: After 7 h of WIR stress, the aortic intima was isolated and prepared the platelet, then NO2- production released from aortic intima and platelet was measured with Greiss regent, NOS activity and L-arginine transport activity were detected by isotope tracer method. RESULTS: After 7 h of WIR stress, the levels of NO2- from platelet and aortic intima obviously decreased by 57% and 46% respectively as compared with the control rats (P<0.01). The inhibitory effects of NO2- showed a positive correlation between platelet and aortic intima by person regression analysis (r2=0.9835, P<0.01). The kinetics analysis of NOS activity of platelet showed that the enzymatic catalytic maximum velocity (Vmax) decreased by 44%, Km values increase by 18% and the enzymatic catalytic efficiency was obviously reduced in the WIR stress rats (P<0.01). The aortic intima NOS activity was inhibited by 50% after WIR stress (P<0.01). The person analysis showed the obvious correlation between them (r2=0.9726, P<0.01). In the WIR stress platelet, the L-Arg transport Vmax obviously reduced by 32%, Km parameter increased 37%, and the transport efficiency was inhibited by 50% (P<0.01), which exhibited obvious positive correlation with the inhibition of L-Arg transport activity in aortic intima (27%) of WIR stress rats (r2=0.9887, P<0.01). CONCLUSION: WIR stress down-regulated endogenous L-Arg/NOS/NO pathway of aortic intima and platelet, whose alteration showed obvious positive correlation. Detection of the alteration of endogenous L-Arg/NOS/NO pathway in platelet might act as an indirect method to assess the endothelial dysfunction involving the pathogensis of stress.

DrugBank Data that Cites this Article

Drug Targets

Drug	Target	Kind	Organism	Pharmacological Action	Actions
Arginine	Nitric oxide synthase, inducible	Protein	Humans	Unknown	Not Available	Details