Cytochrome P450 2E1
Details
- Name
- Cytochrome P450 2E1
- Synonyms
- 1.14.13.-
- 4-nitrophenol 2-hydroxylase
- CYP2E
- CYPIIE1
- Cytochrome P450-J
- Gene Name
- CYP2E1
- Organism
- Humans
- Amino acid sequence
>lcl|BSEQ0012460|Cytochrome P450 2E1 MSALGVTVALLVWAAFLLLVSMWRQVHSSWNLPPGPFPLPIIGNLFQLELKNIPKSFTRL AQRFGPVFTLYVGSQRMVVMHGYKAVKEALLDYKDEFSGRGDLPAFHAHRDRGIIFNNGP TWKDIRRFSLTTLRNYGMGKQGNESRIQREAHFLLEALRKTQGQPFDPTFLIGCAPCNVI ADILFRKHFDYNDEKFLRLMYLFNENFHLLSTPWLQLYNNFPSFLHYLPGSHRKVIKNVA EVKEYVSERVKEHHQSLDPNCPRDLTDCLLVEMEKEKHSAERLYTMDGITVTVADLFFAG TETTSTTLRYGLLILMKYPEIEEKLHEEIDRVIGPSRIPAIKDRQEMPYMDAVVHEIQRF ITLVPSNLPHEATRDTIFRGYLIPKGTVVVPTLDSVLYDNQEFPDPEKFKPEHFLNENGK FKYSDYFKPFSTGKRVCAGEGLARMELFLLLCAILQHFNLKPLVDPKDIDLSPIHIGFGC IPPRYKLCVIPRS
- Number of residues
- 493
- Molecular Weight
- 56848.42
- Theoretical pI
- 8.22
- GO Classification
- Functionsarachidonic acid epoxygenase activity / enzyme binding / heme binding / iron ion binding / monooxygenase activity / oxidoreductase activity / oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen / oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen / oxygen binding / steroid hydroxylase activityProcessesdrug metabolic process / epoxygenase P450 pathway / exogenous drug catabolic process / heterocycle metabolic process / monoterpenoid metabolic process / oxidation-reduction process / response to ethanol / response to organonitrogen compound / response to ozone / small molecule metabolic process / steroid metabolic process / triglyceride metabolic process / xenobiotic metabolic processComponentscytoplasm / endoplasmic reticulum membrane / Golgi membrane / intracellular membrane-bounded organelle / intrinsic component of endoplasmic reticulum membrane / mitochondrion
- General Function
- Steroid hydroxylase activity
- Specific Function
- Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms.
- Pfam Domain Function
- p450 (PF00067)
- Transmembrane Regions
- Not Available
- Cellular Location
- Endoplasmic reticulum membrane
- Gene sequence
>lcl|BSEQ0012461|Cytochrome P450 2E1 (CYP2E1) ATGTCTGCCCTCGGAGTCACCGTGGCCCTGCTGGTGTGGGCGGCCTTCCTCCTGCTGGTG TCCATGTGGAGGCAGGTGCACAGCAGCTGGAATCTGCCCCCAGGCCCTTTCCCGCTTCCC ATCATCGGGAACCTCTTCCAGTTGGAATTGAAGAATATTCCCAAGTCCTTCACCCGGTTG GCCCAGCGCTTCGGGCCGGTGTTCACGCTGTACGTGGGCTCGCAGCGCATGGTGGTGATG CACGGCTACAAGGCGGTGAAGGAAGCGCTGCTGGACTACAAGGACGAGTTCTCGGGCAGA GGCGACCTCCCCGCGTTCCATGCGCACAGGGACAGGGGAATCATTTTTAATAATGGACCT ACCTGGAAGGACATCCGGCGGTTTTCCCTGACCACCCTCCGGAACTATGGGATGGGGAAA CAGGGCAATGAGAGCCGGATCCAGAGGGAGGCCCACTTCCTGCTGGAAGCACTCAGGAAG ACCCAAGGCCAGCCTTTCGACCCCACCTTCCTCATCGGCTGCGCGCCCTGCAACGTCATA GCCGACATCCTCTTCCGCAAGCATTTTGACTACAATGATGAGAAGTTTCTAAGGCTGATG TATTTGTTTAATGAGAACTTCCACCTACTCAGCACTCCCTGGCTCCAGCTTTACAATAAT TTTCCCAGCTTTCTACACTACTTGCCTGGAAGCCACAGAAAAGTCATAAAAAATGTGGCT GAAGTAAAAGAGTATGTGTCTGAAAGGGTGAAGGAGCACCATCAATCTCTGGACCCCAAC TGTCCCCGGGACCTCACCGACTGCCTGCTCGTGGAAATGGAGAAGGAAAAGCACAGTGCA GAGCGCTTGTACACAATGGACGGTATCACCGTGACTGTGGCCGACCTGTTCTTTGCGGGG ACAGAGACCACCAGCACAACTCTGAGATATGGGCTCCTGATTCTCATGAAATACCCTGAG ATCGAAGAGAAGCTCCATGAAGAAATTGACAGGGTGATTGGGCCAAGCCGAATCCCTGCC ATCAAGGATAGGCAAGAGATGCCCTACATGGATGCTGTGGTGCATGAGATTCAGCGGTTC ATCACCCTCGTGCCCTCCAACCTGCCCCATGAAGCAACCCGAGACACCATTTTCAGAGGA TACCTCATCCCCAAGGGCACAGTCGTAGTGCCAACTCTGGACTCTGTTTTGTATGACAAC CAAGAATTTCCTGATCCAGAAAAGTTTAAGCCAGAACACTTCCTGAATGAAAATGGAAAG TTCAAGTACAGTGACTATTTCAAGCCATTTTCCACAGGAAAACGAGTGTGTGCTGGAGAA GGCCTGGCTCGCATGGAGTTGTTTCTTTTGTTGTGTGCCATTTTGCAGCATTTTAATTTG AAGCCTCTCGTTGACCCAAAGGATATCGACCTCAGCCCTATACATATTGGGTTTGGCTGT ATCCCACCACGTTACAAACTCTGTGTCATTCCCCGCTCATGA
- Chromosome Location
- 10
- Locus
- 10q24.3-qter
- External Identifiers
Resource Link UniProtKB ID P05181 UniProtKB Entry Name CP2E1_HUMAN GenBank Protein ID 181360 GenBank Gene ID J02625 HGNC ID HGNC:2631 - General References
- Song BJ, Gelboin HV, Park SS, Yang CS, Gonzalez FJ: Complementary DNA and protein sequences of ethanol-inducible rat and human cytochrome P-450s. Transcriptional and post-transcriptional regulation of the rat enzyme. J Biol Chem. 1986 Dec 15;261(35):16689-97. [Article]
- Umeno M, McBride OW, Yang CS, Gelboin HV, Gonzalez FJ: Human ethanol-inducible P450IIE1: complete gene sequence, promoter characterization, chromosome mapping, and cDNA-directed expression. Biochemistry. 1988 Dec 13;27(25):9006-13. [Article]
- Deloukas P, Earthrowl ME, Grafham DV, Rubenfield M, French L, Steward CA, Sims SK, Jones MC, Searle S, Scott C, Howe K, Hunt SE, Andrews TD, Gilbert JG, Swarbreck D, Ashurst JL, Taylor A, Battles J, Bird CP, Ainscough R, Almeida JP, Ashwell RI, Ambrose KD, Babbage AK, Bagguley CL, Bailey J, Banerjee R, Bates K, Beasley H, Bray-Allen S, Brown AJ, Brown JY, Burford DC, Burrill W, Burton J, Cahill P, Camire D, Carter NP, Chapman JC, Clark SY, Clarke G, Clee CM, Clegg S, Corby N, Coulson A, Dhami P, Dutta I, Dunn M, Faulkner L, Frankish A, Frankland JA, Garner P, Garnett J, Gribble S, Griffiths C, Grocock R, Gustafson E, Hammond S, Harley JL, Hart E, Heath PD, Ho TP, Hopkins B, Horne J, Howden PJ, Huckle E, Hynds C, Johnson C, Johnson D, Kana A, Kay M, Kimberley AM, Kershaw JK, Kokkinaki M, Laird GK, Lawlor S, Lee HM, Leongamornlert DA, Laird G, Lloyd C, Lloyd DM, Loveland J, Lovell J, McLaren S, McLay KE, McMurray A, Mashreghi-Mohammadi M, Matthews L, Milne S, Nickerson T, Nguyen M, Overton-Larty E, Palmer SA, Pearce AV, Peck AI, Pelan S, Phillimore B, Porter K, Rice CM, Rogosin A, Ross MT, Sarafidou T, Sehra HK, Shownkeen R, Skuce CD, Smith M, Standring L, Sycamore N, Tester J, Thorpe A, Torcasso W, Tracey A, Tromans A, Tsolas J, Wall M, Walsh J, Wang H, Weinstock K, West AP, Willey DL, Whitehead SL, Wilming L, Wray PW, Young L, Chen Y, Lovering RC, Moschonas NK, Siebert R, Fechtel K, Bentley D, Durbin R, Hubbard T, Doucette-Stamm L, Beck S, Smith DR, Rogers J: The DNA sequence and comparative analysis of human chromosome 10. Nature. 2004 May 27;429(6990):375-81. [Article]
- Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, Ko MS, Kawakami K, Suzuki Y, Sugano S, Gruber CE, Smith MR, Simmons B, Moore T, Waterman R, Johnson SL, Ruan Y, Wei CL, Mathavan S, Gunaratne PH, Wu J, Garcia AM, Hulyk SW, Fuh E, Yuan Y, Sneed A, Kowis C, Hodgson A, Muzny DM, McPherson J, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madari A, Young AC, Wetherby KD, Granite SJ, Kwong PN, Brinkley CP, Pearson RL, Bouffard GG, Blakesly RW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Griffith M, Griffith OL, Krzywinski MI, Liao N, Morin R, Palmquist D, Petrescu AS, Skalska U, Smailus DE, Stott JM, Schnerch A, Schein JE, Jones SJ, Holt RA, Baross A, Marra MA, Clifton S, Makowski KA, Bosak S, Malek J: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004 Oct;14(10B):2121-7. [Article]
- Lasker JM, Raucy J, Kubota S, Bloswick BP, Black M, Lieber CS: Purification and characterization of human liver cytochrome P-450-ALC. Biochem Biophys Res Commun. 1987 Oct 14;148(1):232-8. [Article]
- Robinson RC, Shorr RG, Varrichio A, Park SS, Gelboin HV, Miller H, Friedman FK: Human liver cytochrome P-450 related to a rat acetone-inducible, nitrosamine-metabolizing cytochrome P-450: identification and isolation. Pharmacology. 1989;39(3):137-44. [Article]
- Gillam EM, Guo Z, Guengerich FP: Expression of modified human cytochrome P450 2E1 in Escherichia coli, purification, and spectral and catalytic properties. Arch Biochem Biophys. 1994 Jul;312(1):59-66. [Article]
- Zerilli A, Ratanasavanh D, Lucas D, Goasduff T, Dreano Y, Menard C, Picart D, Berthou F: Both cytochromes P450 2E1 and 3A are involved in the O-hydroxylation of p-nitrophenol, a catalytic activity known to be specific for P450 2E1. Chem Res Toxicol. 1997 Oct;10(10):1205-12. [Article]
- Bian Y, Song C, Cheng K, Dong M, Wang F, Huang J, Sun D, Wang L, Ye M, Zou H: An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics. 2014 Jan 16;96:253-62. doi: 10.1016/j.jprot.2013.11.014. Epub 2013 Nov 22. [Article]
- Porubsky PR, Meneely KM, Scott EE: Structures of human cytochrome P-450 2E1. Insights into the binding of inhibitors and both small molecular weight and fatty acid substrates. J Biol Chem. 2008 Nov 28;283(48):33698-707. doi: 10.1074/jbc.M805999200. Epub 2008 Sep 24. [Article]
- Hu Y, Oscarson M, Johansson I, Yue QY, Dahl ML, Tabone M, Arinco S, Albano E, Ingelman-Sundberg M: Genetic polymorphism of human CYP2E1: characterization of two variant alleles. Mol Pharmacol. 1997 Mar;51(3):370-6. [Article]
- Fairbrother KS, Grove J, de Waziers I, Steimel DT, Day CP, Crespi CL, Daly AK: Detection and characterization of novel polymorphisms in the CYP2E1 gene. Pharmacogenetics. 1998 Dec;8(6):543-52. [Article]
- Solus JF, Arietta BJ, Harris JR, Sexton DP, Steward JQ, McMunn C, Ihrie P, Mehall JM, Edwards TL, Dawson EP: Genetic variation in eleven phase I drug metabolism genes in an ethnically diverse population. Pharmacogenomics. 2004 Oct;5(7):895-931. [Article]
Drug Relations
- Drug Relations
DrugBank ID Name Drug group Pharmacological action? Actions Details DB00184 Nicotine approved unknown substrateinhibitorinducer Details DB00228 Enflurane approved, investigational, vet_approved unknown substrate Details DB00356 Chlorzoxazone approved unknown substrateinhibitor Details DB00753 Isoflurane approved, vet_approved unknown substrate Details DB00851 Dacarbazine approved, investigational unknown substrate Details DB00898 Ethanol approved unknown substrateinducer Details DB01028 Methoxyflurane approved, investigational, vet_approved, withdrawn unknown substrate Details DB01159 Halothane approved, vet_approved unknown substrate Details DB01204 Mitoxantrone approved, investigational unknown substrateinducer Details DB01213 Fomepizole approved, vet_approved unknown substrateinhibitorinducer Details DB01236 Sevoflurane approved, vet_approved no substrate Details DB00593 Ethosuximide approved unknown substrate Details DB00118 Ademetionine approved, investigational, nutraceutical unknown inhibitor Details DB00277 Theophylline approved unknown substrate Details DB00636 Clofibrate approved, investigational unknown inducer Details DB00780 Phenelzine approved unknown inducer Details DB00908 Quinidine approved, investigational no substrate Details DB00951 Isoniazid approved, investigational unknown substrateinhibitorinducer Details DB01115 Nifedipine approved unknown inhibitor Details DB01173 Orphenadrine approved unknown inhibitor Details DB00250 Dapsone approved, investigational unknown substrate Details DB04977 Plitidepsin investigational unknown substrate Details DB05708 GTS-21 investigational unknown substrate Details DB00316 Acetaminophen approved unknown substrate Details DB00468 Quinine approved unknown substrate Details DB05109 Trabectedin approved, investigational unknown substrate Details DB06728 Aniline experimental unknown substrate Details DB00822 Disulfiram approved unknown inhibitor Details DB01136 Carvedilol approved, investigational unknown substrate Details DB00201 Caffeine approved no substrate Details DB00818 Propofol approved, investigational, vet_approved unknown inhibitor Details DB00969 Alosetron approved, withdrawn unknown inhibitor Details DB01223 Aminophylline approved unknown substrate Details DB00972 Azelastine approved unknown inhibitor Details DB04794 Bifonazole approved, investigational unknown inhibitor Details DB01558 Bromazepam approved, illicit, investigational unknown inhibitor Details DB01156 Bupropion approved unknown substrate Details DB00477 Chlorpromazine approved, investigational, vet_approved unknown inhibitor Details DB00501 Cimetidine approved, investigational unknown inhibitor Details DB00257 Clotrimazole approved, vet_approved unknown inhibitor Details DB01394 Colchicine approved unknown inducer Details DB01151 Desipramine approved, investigational unknown inhibitor Details DB01234 Dexamethasone approved, investigational, vet_approved unknown inducer Details DB01191 Dexfenfluramine approved, illicit, investigational, withdrawn unknown inhibitor Details DB00586 Diclofenac approved, vet_approved unknown inhibitor Details DB01127 Econazole approved no inhibitor Details DB00655 Estrone approved unknown substrate Details DB00773 Etoposide approved unknown substrate Details DB01628 Etoricoxib approved, investigational unknown inhibitor Details DB00949 Felbamate approved unknown substrate Details DB00623 Fluphenazine approved unknown inhibitor Details DB00690 Flurazepam approved, illicit, investigational unknown inhibitor Details DB09462 Glycerin approved, investigational unknown inducer Details DB00458 Imipramine approved unknown inhibitor Details DB00883 Isosorbide dinitrate approved, investigational unknown inhibitor Details DB01167 Itraconazole approved, investigational no inhibitor Details DB01403 Methotrimeprazine approved, investigational unknown inhibitor Details DB01011 Metyrapone approved, investigational unknown inhibitor Details DB00379 Mexiletine approved, investigational unknown substrate Details DB01110 Miconazole approved, investigational, vet_approved unknown inhibitor Details DB00622 Nicardipine approved, investigational unknown substrate Details DB02701 Nicotinamide approved, investigational unknown substrateinhibitor Details DB06712 Nilvadipine approved, investigational unknown substrate Details DB00540 Nortriptyline approved unknown inhibitor Details DB00904 Ondansetron approved, withdrawn unknown substrate Details DB03783 Phenacetin withdrawn unknown substrate Details DB01435 Antipyrine approved, investigational unknown substrate Details DB01174 Phenobarbital approved, investigational unknown substrateinducer Details DB01045 Rifampicin approved unknown inducer Details DB00203 Sildenafil approved, investigational unknown substrateinhibitor Details DB00675 Tamoxifen approved unknown substrate Details DB01079 Tegaserod approved, investigational, withdrawn unknown inhibitor Details DB01412 Theobromine investigational unknown substrate Details DB00763 Methimazole approved unknown inhibitor Details DB00679 Thioridazine approved, withdrawn unknown inhibitor Details DB00208 Ticlopidine approved unknown inhibitor Details DB01007 Tioconazole approved unknown inhibitor Details DB00347 Trimethadione approved unknown substrate Details DB00617 Paramethadione approved unknown substrate Details DB04946 Iloperidone approved unknown substrate Details DB00255 Diethylstilbestrol approved, investigational, withdrawn unknown inhibitor Details DB00918 Almotriptan approved, investigational unknown substrate Details DB06770 Benzyl alcohol approved unknown substrate Details DB06774 Capsaicin approved unknown substrate Details DB04920 Clevidipine approved, investigational unknown substrate Details DB00633 Dexmedetomidine approved, vet_approved unknown substrate Details DB06370 Indisulam investigational unknown inhibitor Details DB00109 Enfuvirtide approved, investigational unknown substrate Details DB00041 Aldesleukin approved unknown inhibitor Details DB04858 Tirapazamine investigational unknown substrate Details DB00428 Streptozocin approved, investigational unknown inducer Details DB06637 Dalfampridine approved unknown substrate Details DB08868 Fingolimod approved, investigational unknown substrate Details DB06201 Rufinamide approved unknown inhibitor Details DB01068 Clonazepam approved, illicit unknown inhibitor Details DB01544 Flunitrazepam approved, illicit unknown inhibitor Details DB01595 Nitrazepam approved unknown inhibitor Details DB08846 Ellagic acid investigational unknown inhibitor Details DB06595 Midostaurin approved, investigational unknown inhibitor Details DB13174 Rhein experimental unknown inhibitor Details DB11994 Diacerein approved, investigational unknown inhibitor Details DB11943 Delafloxacin approved, investigational unknown inducer Details DB09120 Zucapsaicin approved, investigational unknown inhibitor Details DB13941 Piperaquine approved, experimental, investigational unknown inducer Details DB09328 Vayarin approved, investigational unknown inhibitor Details DB00486 Nabilone approved, investigational no substrateinhibitor Details DB04379 N-Methyl-N-(Methylbenzyl)Formamide experimental unknown substrate Details DB01154 Thiamylal approved, vet_approved no substrate Details DB09256 Tegafur approved, investigational unknown substrate Details DB09288 Propacetamol experimental no substrate Details DB14046 Dichlorobenzene approved, experimental unknown substrate Details DB13963 1,2-dichlorobenzene experimental unknown substrate Details DB01844 N,N-dimethylformamide experimental unknown substrate Details DB02520 Ditiocarb experimental unknown inhibitor Details DB00627 Niacin approved, investigational, nutraceutical unknown inhibitor Details DB04856 Dexloxiglumide investigational unknown substrate Details DB00661 Verapamil approved unknown substrate Details DB06119 Cenobamate approved, investigational unknown substrate Details DB01104 Sertraline approved unknown substrate Details DB00252 Phenytoin approved, vet_approved no substrate Details DB00794 Primidone approved, vet_approved unknown substrateinducer Details DB00869 Dorzolamide approved no substrate Details DB13609 Umifenovir investigational unknown substrate Details DB11837 Osilodrostat approved, investigational unknown inhibitor Details DB12466 Favipiravir approved, investigational unknown inhibitor Details DB11689 Selumetinib approved, investigational no substrate Details DB14840 Ripretinib approved unknown Details DB14649 Dexamethasone acetate approved, investigational, vet_approved unknown inducer Details DB12499 Clascoterone approved, investigational no inhibitor Details DB06448 Lonafarnib approved, investigational no substrate Details DB04951 Pirfenidone approved, investigational unknown substrate Details DB01189 Desflurane approved no substrate Details DB00170 Menadione approved, nutraceutical unknown inhibitor Details DB08864 Rilpivirine approved unknown inhibitor Details DB00330 Ethambutol approved unknown inhibitor Details DB01086 Benzocaine approved, investigational unknown substrate Details DB08995 Diosmin approved, investigational unknown inhibitor Details DB00412 Rosiglitazone approved, investigational unknown substrate Details DB00602 Ivermectin approved, investigational, vet_approved no substrate Details DB04216 Quercetin experimental, investigational no inhibitor Details DB09061 Cannabidiol approved, investigational no substrateinhibitor Details DB14737 Cannabinol experimental, investigational unknown inhibitor Details DB14009 Medical Cannabis experimental, investigational unknown substrateinhibitor Details DB06016 Cariprazine approved, investigational no inhibitor Details DB00755 Tretinoin approved, investigational, nutraceutical unknown substrate Details